A cuvette is a small tube of circular or square cross section, sealed at one end and designed to hold samples for spectroscopic experiments. Disposable plastic cuvettes are often used in fast spectroscopic assays.

Some cuvettes will be clear only on opposite sides, so that they pass a single beam of light through that pair of sides; often the unclear sides have ridges or are rough to allow easy handling. Cuvettes to be used in fluorescence spectroscopy must be clear on all four sides because fluorescence is measured at a right-angle to the beam path to limit contributions from beam itself. Typically, cuvettes are 10 mm (0.39 in) across, to allow for easy calculations of coefficients of absorption.

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